Journal: Bio-protocol
Article Title: Introducing Exogenous DNA Vectors Directly into Trypoxylus dichotomus Larvae Via In Vivo Electroporation
doi: 10.21769/BioProtoc.5602
Figure Lengend Snippet: (A) GDC-1 glass capillary and GDC-1 glass capillary with a scale added in 0.6 mm increments. The inner diameter of the GDC-1 glass capillary is 0.6 mm, yielding approximately 0.17 μL per scale division (0.3 mm × 0.3 mm × π × 0.6 mm ≈ 0.17 μL). (B) The tip of a glass capillary produced with a micropipette puller is sealed (green). Under a stereomicroscope, the capillary tip should be broken with tweezers to obtain a suitably narrow aperture (magenta). If the aperture is excessively wide, it may result in larval mortality (gray). Additionally, sharpening the tip of the broken glass capillary with a micropipette grinder can reduce mechanical damage to the larva (blue).
Article Snippet: Micropipette [Gilson, catalog number: F144054M (P2), F144056M (P20)] 2.
Techniques: Produced